Journal: Frontiers in Pediatrics

Article Title: Glucose-6-Phosphate Dehydrogenase Deficiency and Neonatal Hyperbilirubinemia: Insights on Pathophysiology, Diagnosis, and Gene Variants in Disease Heterogeneity

doi: 10.3389/fped.2022.875877

Figure Lengend Snippet: The three RBC antioxidant systems that are compromised in G6PD deficiency. The more familiar glutathione peroxidase/glutathione reductase (GPx/GR) and antioxidant mechanisms are shown in gray (middle) and the more recently recognized peroxiredoxin 2 system in light blue (top). Prdx2 is a thiol protein that is oxidized to an interchain disulfide then recycled predominantly by thioredoxin (Trx) and thioredoxin reductase [TrxR; ]. Prdx2, the third most abundant RBC protein, is present at a much higher concentration than glutathione peroxidase or catalase. It is highly reactive with peroxides and is favored to consume most of the intracellular H 2 O 2 . NADPH is required as a reducing substrate for both GR and TrxR, and it protects catalase against inactivation. It is produced via the pentose phosphate pathway of which the first step is catalyzed by G6PD. By restricting the supply of NADPH, G6PD deficiency compromises the ability of all three antioxidant systems to detoxify hydrogen peroxide. O 2 – , superoxide; H 2 O 2 , hydrogen peroxide; GSH, reduced glutathione; GSSG, glutathione disulfide; SOD, superoxide dismutase. The “red” and “ox” subscripts refer, respectively, to the reduced and oxidized forms of the proteins.

Article Snippet: The G6PD Deficiency GenoArray Diagnostic Kit (Hybribio, Sheung Wan, Hong Kong, China) is an all-in-one integrated polymerase chain reaction (PCR), hybridization, and gene chip processing system based on flow-through hybridization technology which can provide a rapid and accurate nucleic acid analysis of a patient sample.

Techniques: Concentration Assay, Produced

Journal: Frontiers in Pediatrics

Article Title: Glucose-6-Phosphate Dehydrogenase Deficiency and Neonatal Hyperbilirubinemia: Insights on Pathophysiology, Diagnosis, and Gene Variants in Disease Heterogeneity

doi: 10.3389/fped.2022.875877

Figure Lengend Snippet: Kinetic parameters in G6PD enzyme activity between some genetic variants against G6PD B (normal).

Article Snippet: The G6PD Deficiency GenoArray Diagnostic Kit (Hybribio, Sheung Wan, Hong Kong, China) is an all-in-one integrated polymerase chain reaction (PCR), hybridization, and gene chip processing system based on flow-through hybridization technology which can provide a rapid and accurate nucleic acid analysis of a patient sample.

Techniques: Activity Assay

Journal: Frontiers in Pediatrics

Article Title: Glucose-6-Phosphate Dehydrogenase Deficiency and Neonatal Hyperbilirubinemia: Insights on Pathophysiology, Diagnosis, and Gene Variants in Disease Heterogeneity

doi: 10.3389/fped.2022.875877

Figure Lengend Snippet: Specific G6PD variants that have been reported to be associated with neonatal hyperbilirubinemia.

Article Snippet: The G6PD Deficiency GenoArray Diagnostic Kit (Hybribio, Sheung Wan, Hong Kong, China) is an all-in-one integrated polymerase chain reaction (PCR), hybridization, and gene chip processing system based on flow-through hybridization technology which can provide a rapid and accurate nucleic acid analysis of a patient sample.

Techniques:

Journal: Frontiers in Pediatrics

Article Title: Glucose-6-Phosphate Dehydrogenase Deficiency and Neonatal Hyperbilirubinemia: Insights on Pathophysiology, Diagnosis, and Gene Variants in Disease Heterogeneity

doi: 10.3389/fped.2022.875877

Figure Lengend Snippet: A crystal violet supravital staining of a peripheral blood film, viewed at 40x magnification. Note the absence of Heinz bodies in this blood film from a G6PD-deficient neonate with hyperbilirubinemia.

Article Snippet: The G6PD Deficiency GenoArray Diagnostic Kit (Hybribio, Sheung Wan, Hong Kong, China) is an all-in-one integrated polymerase chain reaction (PCR), hybridization, and gene chip processing system based on flow-through hybridization technology which can provide a rapid and accurate nucleic acid analysis of a patient sample.

Techniques: Staining

Journal: Frontiers in Pediatrics

Article Title: Glucose-6-Phosphate Dehydrogenase Deficiency and Neonatal Hyperbilirubinemia: Insights on Pathophysiology, Diagnosis, and Gene Variants in Disease Heterogeneity

doi: 10.3389/fped.2022.875877

Figure Lengend Snippet: Quantitative Polymerase Chain Reaction (qPCR) for G6PD variant analysis in a newborn infant with early onset jaundice. (A) This assay confirmed that the female infant with severe hyperbilirubinemia at day 3 of life but with G6PD enzyme activity reported within the normal reference range, was heterozygous for G6PD Mahidol (c.487G > A). DNA sequencing result is shown in (B) .

Article Snippet: The G6PD Deficiency GenoArray Diagnostic Kit (Hybribio, Sheung Wan, Hong Kong, China) is an all-in-one integrated polymerase chain reaction (PCR), hybridization, and gene chip processing system based on flow-through hybridization technology which can provide a rapid and accurate nucleic acid analysis of a patient sample.

Techniques: Real-time Polymerase Chain Reaction, Variant Assay, Activity Assay, DNA Sequencing

Journal: Journal of Medical Virology

Article Title: A Narrative Review of the Putative Etiologic Role and Diagnostic Utility of the Cervicovaginal Microbiome in Human Papillomavirus‐Associated Cervical Carcinogenesis

doi: 10.1002/jmv.70027

Figure Lengend Snippet: Mapping of the search strategy, methodology, and results. To assess the role of the CVM in HPV‐associated cervical carcinogenesis, 2223 records were identified in Embase, Medline and Web of Science, of which 951 duplicates were excluded. 1272 articles were screened by title and abstract for relevance and the full texts of 276 articles were assessed for eligibility. Two additional articles were identified in PubMed following the systematic search. A total of 21 observational studies, 4 systematic reviews, and 3 meta‐analyses were included. To assess the diagnostic performance of the CVM in HPV‐associated cervical carcinogenesis, 7 relevant articles were identified in PubMed, of which 3 were excluded. Of the 4 full texts assessed for eligibility, one was excluded as it only assessed the diagnostic performance of bacterial genes (not taxa). The corresponding search strategies for the aforementioned searches are detailed in Table . To identify common metagenomic techniques in microbial research, 11 resources were identified through a focused search of Google and Google Scholar to identify webpages and scholarly articles, respectively. Abbreviations: CVM, cervicovaginal microbiome; HPV, human papillomavirus; ROC, receiver operating characteristic; WMS, whole metagenome sequencing; 16S rRNA, 16S ribosomal RNA.

Article Snippet: [ ] , Cross‐sectional Women attending a colposcopy clinic following screening for cervical cancer ( n = 52; 27 HPV16 + , 25 HPV− ) Ages, 25–42 years , Shotgun metagenomic sequencing NA Hiseq X‐ten platform , Hybribio Rapid GenoArray test kit 15 hrHPVs, 6 lrHPVs hrHPVs: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68, 66, and 53 lrHPVs: 6, 11, 42, 43, 44, and CP8304 , Random forest ensemble learning based on the mean decrease accuracy HPV16+ versus HPV− , 17 genera NR , 0.819 (0.684–0.954).

Techniques: Diagnostic Assay, Sequencing